The condensed protocols from molecular cloning : a laboratory manual /
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| Formato: | Livro |
| Idioma: | inglês |
| Publicado em: |
Cold Spring Harbor, N.Y.
Cold Spring Harbor Laboratory Press
c2006.
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| Acesso em linha: | Table of contents View in OPAC |
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| 100 | 1 | |a Sambrook, Joseph. | |
| 245 | 1 | 4 | |a The condensed protocols from molecular cloning : |b a laboratory manual / |c Joseph Sambrook, David W. Russell. |
| 260 | |a Cold Spring Harbor, N.Y. |b Cold Spring Harbor Laboratory Press |c c2006. | ||
| 263 | |a 1111 | ||
| 300 | |a 800 p.: ill.; 28 cm. | ||
| 500 | |a Includes index. | ||
| 505 | 0 | |a Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies. | |
| 650 | 0 | |a Molecular cloning |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Cloning, Molecular |v Laboratory Manuals. |
| 700 | 1 | |a Russell, David W. |q (David William), |d 1957- | |
| 856 | 4 | 1 | |3 Table of contents |u http://www.loc.gov/catdir/toc/ecip0517/2005022077.html |
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| 955 | |d jc15 2005-07-29 |e jc15 2005-07-29 to Dewey |d jc18 2006-03-03 (re-cuttered) | ||
| 963 | |a Denise Weiss; phone: 516-422-4024; fax: 516-422-4095; email: weiss@cshl.edu; bc: powerse@cshl.edu | ||
| 999 | |c 8221 |d 8221 | ||