Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue

Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38+/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from...

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Autor principal: Simmons, Cameron
Formato: Journal Article
Lenguaje:inglés
Publicado: 2018
Acceso en línea:https://demo7.dspace.org/handle/123456789/121
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author Simmons, Cameron
author_browse Simmons, Cameron
author_facet Simmons, Cameron
author_sort Simmons, Cameron
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description Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38+/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3(133-142)-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3(133-142)-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3(133-142)-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.
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spelling oai:localhost:123456789-1212021-04-07T16:30:07Z Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue Simmons, Cameron Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38+/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3(133-142)-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3(133-142)-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3(133-142)-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF. 2018-09-14T11:14:56Z 2017-07-12T05:11:31Z 2018-09-14T11:14:56Z 2010-06-15 Journal Article https://demo7.dspace.org/handle/123456789/121 English
spellingShingle Simmons, Cameron
Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title_full Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title_fullStr Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title_full_unstemmed Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title_short Timing of CD8(+) T Cell Responses in Relation to Commencement of Capillary Leakage in Children with Dengue
title_sort timing of cd8 t cell responses in relation to commencement of capillary leakage in children with dengue
url https://demo7.dspace.org/handle/123456789/121
work_keys_str_mv AT simmonscameron timingofcd8tcellresponsesinrelationtocommencementofcapillaryleakageinchildrenwithdengue