Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration

Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate that cranial NC (CNC) cells are a homogeneous population relying on cell-cel...

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Hauptverfasser: Richardson, Jo, Gauert, Anton, Montecinos, Luis Briones, Fanlo, Lucía, Alhashem, Zainalabdeen Mohmammed, Assar, Rodrigo, Marti, Elisa, Kabla, Alexandre, Härtel, Steffen, Linker, Claudia
Sprache:Englisch
Veröffentlicht: Elsevier 2019
Online-Zugang:https://demo7.dspace.org/handle/123456789/439
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author Richardson, Jo
Gauert, Anton
Montecinos, Luis Briones
Fanlo, Lucía
Alhashem, Zainalabdeen Mohmammed
Assar, Rodrigo
Marti, Elisa
Kabla, Alexandre
Härtel, Steffen
Linker, Claudia
author_browse Alhashem, Zainalabdeen Mohmammed
Assar, Rodrigo
Fanlo, Lucía
Gauert, Anton
Härtel, Steffen
Kabla, Alexandre
Linker, Claudia
Marti, Elisa
Montecinos, Luis Briones
Richardson, Jo
author_facet Richardson, Jo
Gauert, Anton
Montecinos, Luis Briones
Fanlo, Lucía
Alhashem, Zainalabdeen Mohmammed
Assar, Rodrigo
Marti, Elisa
Kabla, Alexandre
Härtel, Steffen
Linker, Claudia
author_sort Richardson, Jo
collection DSpace
description Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate that cranial NC (CNC) cells are a homogeneous population relying on cell-cell interactions for directional migration, while chick embryo analyses suggest a heterogeneous population with leader cells instructing directionality. Our data in chick and zebrafish embryos show that CNC cells do not require leader cells for migration and all cells present similar migratory capacities. In contrast, laser ablation of trunk NC (TNC) cells shows that leader cells direct movement and cell-cell contacts are required for migration. Moreover, leader and follower identities are acquired before the initiation of migration and remain fixed thereafter. Thus, two distinct mechanisms establish the directionality of CNC cells and TNC cells. This implies the existence of multiple molecular mechanisms for collective cell migration.
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spelling oai:localhost:123456789-4392021-04-07T16:30:11Z Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration Richardson, Jo Gauert, Anton Montecinos, Luis Briones Fanlo, Lucía Alhashem, Zainalabdeen Mohmammed Assar, Rodrigo Marti, Elisa Kabla, Alexandre Härtel, Steffen Linker, Claudia Collective cell migration is fundamental for life and a hallmark of cancer. Neural crest (NC) cells migrate collectively, but the mechanisms governing this process remain controversial. Previous analyses in Xenopus indicate that cranial NC (CNC) cells are a homogeneous population relying on cell-cell interactions for directional migration, while chick embryo analyses suggest a heterogeneous population with leader cells instructing directionality. Our data in chick and zebrafish embryos show that CNC cells do not require leader cells for migration and all cells present similar migratory capacities. In contrast, laser ablation of trunk NC (TNC) cells shows that leader cells direct movement and cell-cell contacts are required for migration. Moreover, leader and follower identities are acquired before the initiation of migration and remain fixed thereafter. Thus, two distinct mechanisms establish the directionality of CNC cells and TNC cells. This implies the existence of multiple molecular mechanisms for collective cell migration. 2019-04-26T08:56:57Z 2019-04-26T08:56:57Z 19/05/16 https://demo7.dspace.org/handle/123456789/439 en Elsevier
spellingShingle Richardson, Jo
Gauert, Anton
Montecinos, Luis Briones
Fanlo, Lucía
Alhashem, Zainalabdeen Mohmammed
Assar, Rodrigo
Marti, Elisa
Kabla, Alexandre
Härtel, Steffen
Linker, Claudia
Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title_full Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title_fullStr Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title_full_unstemmed Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title_short Leader Cells Define Directionality of Trunk, but Not Cranial, Neural Crest Cell Migration
title_sort leader cells define directionality of trunk but not cranial neural crest cell migration
url https://demo7.dspace.org/handle/123456789/439
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