The condensed protocols from molecular cloning : a laboratory manual /
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| Autore principale: | |
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| Altri autori: | |
| Natura: | Libro |
| Lingua: | inglese |
| Pubblicazione: |
Cold Spring Harbor, N.Y.
Cold Spring Harbor Laboratory Press
c2006.
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| Soggetti: | |
| Accesso online: | Table of contents Visualizza in OPAC |
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Sommario:
- Plasmids and their usefulness in molecular cloning
- Bacteriophage and its vectors
- Working with bacteriophage M13
- Working with high-capacity vectors
- Gas electrophoresisof DNA and pulsed-field agarose
- Preparation and analysis of eukaryotic genomic DNA
- Extraction, purification, and analysis of mRNA from eukaryotic cells
- In vitro amplification of DNA by the polymerase chain reaction
- Preparation of radiolabeled DNA and RNA probes
- Working with synthetic oligonucleotide probes
- Preparation of cDNA libraries and gene identification
- DNA sequencing
- Mutagenesis
- Screening expression libraries
- Expression of cloned genes in escherichia coli
- Introducing cloned genes into cultured mammalian cells
- Analysis of gene expression in cultured mammalian cells
- Protein interaction technologies.