miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression

Defining the molecular and cellular roots of lung cancer relapse after initial treatment remains an imperative to improve survival. Here we report that the lung stem cell marker Lgr6 becomes enriched in non-small cell lung cancer (NSCLC) cells during malignant progression. Lgr6+ NSCLC cells displaye...

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Hauptverfasser: Guinot, Anna, Oeztuerk-Winder, Feride, Ventura, Juan-Jose
Sprache:Englisch
Veröffentlicht: American Association for Cancer Research 2019
Online-Zugang:https://demo7.dspace.org/handle/123456789/443
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author Guinot, Anna
Oeztuerk-Winder, Feride
Ventura, Juan-Jose
author_browse Guinot, Anna
Oeztuerk-Winder, Feride
Ventura, Juan-Jose
author_facet Guinot, Anna
Oeztuerk-Winder, Feride
Ventura, Juan-Jose
author_sort Guinot, Anna
collection DSpace
description Defining the molecular and cellular roots of lung cancer relapse after initial treatment remains an imperative to improve survival. Here we report that the lung stem cell marker Lgr6 becomes enriched in non-small cell lung cancer (NSCLC) cells during malignant progression. Lgr6+ NSCLC cells displayed self-renewal and differentiation properties along with a higher tumorigenic potential. Mechanistic investigations suggested that a defective repression of the miR-17-92 gene cluster was responsible for evolution of a selection for outgrowth of Lgr6+ NSCLC cells. High levels of expression of miR-19 family members were found to target and downregulate levels of p38α kinase, providing a specific survival signal for Lgr6+ cells as mediated by increased Wnt/ß-catenin activity. Our results identify a specific stem-like cell population in NSCLC with increased malignant potential, the elucidation of which may enable earlier prognosis and possibly the development of more effective targeted treatments.
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publisher American Association for Cancer Research
publisherStr American Association for Cancer Research
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spelling oai:localhost:123456789-4432021-04-07T16:30:12Z miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression Guinot, Anna Oeztuerk-Winder, Feride Ventura, Juan-Jose Defining the molecular and cellular roots of lung cancer relapse after initial treatment remains an imperative to improve survival. Here we report that the lung stem cell marker Lgr6 becomes enriched in non-small cell lung cancer (NSCLC) cells during malignant progression. Lgr6+ NSCLC cells displayed self-renewal and differentiation properties along with a higher tumorigenic potential. Mechanistic investigations suggested that a defective repression of the miR-17-92 gene cluster was responsible for evolution of a selection for outgrowth of Lgr6+ NSCLC cells. High levels of expression of miR-19 family members were found to target and downregulate levels of p38α kinase, providing a specific survival signal for Lgr6+ cells as mediated by increased Wnt/ß-catenin activity. Our results identify a specific stem-like cell population in NSCLC with increased malignant potential, the elucidation of which may enable earlier prognosis and possibly the development of more effective targeted treatments. 2019-04-26T08:57:01Z 2019-04-26T08:57:01Z 04/05/16 https://demo7.dspace.org/handle/123456789/443 en American Association for Cancer Research
spellingShingle Guinot, Anna
Oeztuerk-Winder, Feride
Ventura, Juan-Jose
miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title_full miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title_fullStr miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title_full_unstemmed miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title_short miR-17-92/p38α dysregulation enhances Wnt signal and selects Lgr6+ cancer stem cell like cells during human lung adenocarcinoma progression
title_sort mir 17 92 p38α dysregulation enhances wnt signal and selects lgr6 cancer stem cell like cells during human lung adenocarcinoma progression
url https://demo7.dspace.org/handle/123456789/443
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AT oeztuerkwinderferide mir1792p38adysregulationenhanceswntsignalandselectslgr6cancerstemcelllikecellsduringhumanlungadenocarcinomaprogression
AT venturajuanjose mir1792p38adysregulationenhanceswntsignalandselectslgr6cancerstemcelllikecellsduringhumanlungadenocarcinomaprogression